Structure and evolution of 4-coumarate:coenzyme A ligase (4CL) gene families.
Identifieur interne : 004653 ( Main/Exploration ); précédent : 004652; suivant : 004654Structure and evolution of 4-coumarate:coenzyme A ligase (4CL) gene families.
Auteurs : D. Cukovic [Canada] ; J. Ehlting ; J A Vanziffle ; C J DouglasSource :
- Biological chemistry [ 1431-6730 ] ; 2001.
Descripteurs français
- KwdFr :
- ADN des plantes (isolement et purification), AMP (métabolisme), ARN des plantes (isolement et purification), Amplification de gène (génétique), Arabidopsis (enzymologie), Arabidopsis (génétique), Coenzyme A ligases (composition chimique), Coenzyme A ligases (génétique), Coenzyme A ligases (métabolisme), Données de séquences moléculaires (MeSH), Petroselinum (enzymologie), Petroselinum (génétique), Phylogenèse (MeSH), Protéines recombinantes (génétique), Réaction de polymérisation en chaîne (MeSH), Séquence d'acides aminés (génétique), Tabac (enzymologie), Tabac (génétique).
- MESH :
- composition chimique : Coenzyme A ligases.
- enzymologie : Arabidopsis, Petroselinum, Tabac.
- génétique : Amplification de gène, Arabidopsis, Coenzyme A ligases, Petroselinum, Protéines recombinantes, Séquence d'acides aminés, Tabac.
- isolement et purification : ADN des plantes, ARN des plantes.
- métabolisme : AMP, Coenzyme A ligases.
- Données de séquences moléculaires, Phylogenèse, Réaction de polymérisation en chaîne.
English descriptors
- KwdEn :
- Adenosine Monophosphate (metabolism), Amino Acid Sequence (genetics), Arabidopsis (enzymology), Arabidopsis (genetics), Coenzyme A Ligases (chemistry), Coenzyme A Ligases (genetics), Coenzyme A Ligases (metabolism), DNA, Plant (isolation & purification), Gene Amplification (genetics), Molecular Sequence Data (MeSH), Petroselinum (enzymology), Petroselinum (genetics), Phylogeny (MeSH), Polymerase Chain Reaction (MeSH), RNA, Plant (isolation & purification), Recombinant Proteins (genetics), Tobacco (enzymology), Tobacco (genetics).
- MESH :
- chemical , chemistry : Coenzyme A Ligases.
- chemical , genetics : Coenzyme A Ligases, Recombinant Proteins.
- chemical , isolation & purification : DNA, Plant, RNA, Plant.
- chemical , metabolism : Adenosine Monophosphate, Coenzyme A Ligases.
- enzymology : Arabidopsis, Petroselinum, Tobacco.
- genetics : Amino Acid Sequence, Arabidopsis, Gene Amplification, Petroselinum, Tobacco.
- Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction.
Abstract
The phenylpropanoid enzyme 4-coumarate:coenzyme A ligase (4CL) plays a key role in general phenylpropanoid metabolism. 4CL is related to a larger class of prokaryotic and eukaryotic adenylate-forming enzymes and shares several conserved peptide motifs with these enzymes. In order to better characterize the nature of 4CL gene families in poplar, parsley, and tobacco, we used degenerate primers to amplify 4CL sequences from these species. In each species additional, divergent 4CL genes were found. Complete cDNA clones for the two new poplar 4CL genes were obtained, allowing examination of their expression patterns and determination of the substrate utilization profile of a xylem-specific isoform. Phylogenetic analysis of these genes and gene fragments confirmed previous results showing that 4CL proteins fall into two evolutionarily ancient subgroups . A comparative phylogenetic analysis of enzymes in the adenylate-forming superfamily showed that 4CLs, luciferases, and acetate CoA ligases each form distinct clades within the superfamily. According to this analysis, four Arabidopsis 4CL-like genes identified from the Arabidopsis Genome Project are only distantly related to bona fide 4CLs or are more closely related to fatty acid CoA ligases, suggesting that the three Arabidopsis 4CL genes previously characterized represent the extent of the 4CL gene family in this species.
DOI: 10.1515/BC.2001.076
PubMed: 11405227
Affiliations:
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Le document en format XML
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In order to better characterize the nature of 4CL gene families in poplar, parsley, and tobacco, we used degenerate primers to amplify 4CL sequences from these species. In each species additional, divergent 4CL genes were found. Complete cDNA clones for the two new poplar 4CL genes were obtained, allowing examination of their expression patterns and determination of the substrate utilization profile of a xylem-specific isoform. Phylogenetic analysis of these genes and gene fragments confirmed previous results showing that 4CL proteins fall into two evolutionarily ancient subgroups . A comparative phylogenetic analysis of enzymes in the adenylate-forming superfamily showed that 4CLs, luciferases, and acetate CoA ligases each form distinct clades within the superfamily. According to this analysis, four Arabidopsis 4CL-like genes identified from the Arabidopsis Genome Project are only distantly related to bona fide 4CLs or are more closely related to fatty acid CoA ligases, suggesting that the three Arabidopsis 4CL genes previously characterized represent the extent of the 4CL gene family in this species.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11405227</PMID><DateCompleted><Year>2001</Year><Month>12</Month><Day>04</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1431-6730</ISSN><JournalIssue CitedMedium="Print"><Volume>382</Volume><Issue>4</Issue><PubDate><Year>2001</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Biological chemistry</Title><ISOAbbreviation>Biol Chem</ISOAbbreviation></Journal><ArticleTitle>Structure and evolution of 4-coumarate:coenzyme A ligase (4CL) gene families.</ArticleTitle><Pagination><MedlinePgn>645-54</MedlinePgn></Pagination><Abstract><AbstractText>The phenylpropanoid enzyme 4-coumarate:coenzyme A ligase (4CL) plays a key role in general phenylpropanoid metabolism. 4CL is related to a larger class of prokaryotic and eukaryotic adenylate-forming enzymes and shares several conserved peptide motifs with these enzymes. In order to better characterize the nature of 4CL gene families in poplar, parsley, and tobacco, we used degenerate primers to amplify 4CL sequences from these species. In each species additional, divergent 4CL genes were found. Complete cDNA clones for the two new poplar 4CL genes were obtained, allowing examination of their expression patterns and determination of the substrate utilization profile of a xylem-specific isoform. Phylogenetic analysis of these genes and gene fragments confirmed previous results showing that 4CL proteins fall into two evolutionarily ancient subgroups . A comparative phylogenetic analysis of enzymes in the adenylate-forming superfamily showed that 4CLs, luciferases, and acetate CoA ligases each form distinct clades within the superfamily. According to this analysis, four Arabidopsis 4CL-like genes identified from the Arabidopsis Genome Project are only distantly related to bona fide 4CLs or are more closely related to fatty acid CoA ligases, suggesting that the three Arabidopsis 4CL genes previously characterized represent the extent of the 4CL gene family in this species.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cukovic</LastName><ForeName>D</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Botany, University of British Columbia, Vancouver, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ehlting</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>VanZiffle</LastName><ForeName>J A</ForeName><Initials>JA</Initials></Author><Author ValidYN="Y"><LastName>Douglas</LastName><ForeName>C J</ForeName><Initials>CJ</Initials></Author></AuthorList><Language>eng</Language><DataBankList 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PubStatus="medline"><Year>2002</Year><Month>1</Month><Day>5</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2001</Year><Month>6</Month><Day>19</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11405227</ArticleId><ArticleId IdType="doi">10.1515/BC.2001.076</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Colombie-Britannique</li></region><settlement><li>Vancouver</li></settlement><orgName><li>Université de la Colombie-Britannique</li></orgName></list><tree><noCountry><name sortKey="Douglas, C J" sort="Douglas, C J" uniqKey="Douglas C" first="C J" last="Douglas">C J Douglas</name><name sortKey="Ehlting, J" sort="Ehlting, J" uniqKey="Ehlting J" first="J" last="Ehlting">J. Ehlting</name><name sortKey="Vanziffle, J A" sort="Vanziffle, J A" uniqKey="Vanziffle J" first="J A" last="Vanziffle">J A Vanziffle</name></noCountry><country name="Canada"><region name="Colombie-Britannique"><name sortKey="Cukovic, D" sort="Cukovic, D" uniqKey="Cukovic D" first="D" last="Cukovic">D. Cukovic</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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